Quantitative Community Fingerprinting Methods for Estimating the Abundance of Operational Taxonomic Units in Natural Microbial Communities
نویسندگان
چکیده
منابع مشابه
Quantitative community fingerprinting methods for estimating the abundance of operational taxonomic units in natural microbial communities.
Molecular fingerprinting techniques offer great promise for analyzing changes in microbial community structure, especially when dealing with large number of samples. However, a serious limitation has been the lack of quantification offered by such techniques since the relative abundances of the identified operational taxonomic units (OTUs) in the original samples are not measured. A quantitativ...
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DNA sequence information has increasingly been used in ecological research on microbial eukaryotes. Sequence-based approaches have included studies of the total diversity of selected ecosystems, studies of the autecology of ecologically relevant species, and identification and enumeration of species of interest for human health. It is still uncommon, however, to delineate protistan species base...
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Difficulties in cultivating most of the microorganisms limit our ability to study microbial ecosystems. Molecular methods are valuable tools for investigating the diversity and structure of bacterial communities. These techniques can be used on culturable as well as non-culturable bacteria. Cultivation independent techniques based on nucleic acids extracted from the environment provide informat...
متن کاملErratum to: Stability of operational taxonomic units: an important but neglected property for analyzing microbial diversity
[This corrects the article DOI: 10.1186/s40168-015-0081-x.].
متن کاملStability of operational taxonomic units: an important but neglected property for analyzing microbial diversity
BACKGROUND The operational taxonomic unit (OTU) is widely used in microbial ecology. Reproducibility in microbial ecology research depends on the reliability of OTU-based 16S ribosomal subunit RNA (rRNA) analyses. RESULTS Here, we report that many hierarchical and greedy clustering methods produce unstable OTUs, with membership that depends on the number of sequences clustered. If OTUs are re...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2009
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.02409-08